Coding

Part:BBa_K1583104:Design

Designed by: Stefan Robert Marsden   Group: iGEM15_TU_Delft   (2015-09-04)


pRha + Mfp5_CsgA_His fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 245
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A mutation had to be introduced at base pair 766 to remove a PstI restriction site. The protein sequence was not changed in doing so.


Source

This part originates from the E.coli K-12 MG1655 genome and from mussels. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1]

1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.


References